ANTIBACTERIAL ACTIVITY AND BIOAUTOGRAPHIC EVALUATION OF EXTRACT AND FRACTION FROM TAMOENJU (HIBISCUS SURATTENSIS L.) LEAVES

Objective: The tamoenju (Hibiscus surattensis L.) is one of the plants as traditional medicines to treat infections. Tamoenju leaves contain alkaloids, flavonoids, saponins, tannins, and steroids, a potential antibacterial agent. This study aimed determine activity extract fraction against Staphylococcus aureus (ATCC 25923) Salmonella typhi 14028), detect active compounds using Thin Layer Chromatography (TLC) Bioautography techniques.
 Methods: sample was extracted maceration method with 96% ethanol solvent. Fractionation liquid-liquid extraction n-hexane ethyl acetate. agar well diffusion used evaluate various concentrations 2.5%, 5%, 10%, 20%, followed by TLC bioautography n-butanol: acetic acid: aquadest (4:1:1) mobile phase silica gel GF 254 stationary on most fraction. Zones inhibition showed sensitivity tested microorganisms.
 Results: results extract, n-hexane, water fractions were more sensitive S. typhi, while acetate both bacteria. zone increased increasing concentration. that had in (ethyl fraction) flavonoids.
 Conclusion: have activity. Ethyl highest predicted two bacteria flavonoids.